Fig. 4.

Effect of apigenin administration on the expression of Ki-67, cyclin D1, p27/Kip1 and BIM in the dorsolateral prostate of TRAMP mice. Apigenin was administered at 20 and 50 μg/mouse/day (wt/vol) by gavage in 0.2 ml of a vehicle consisting of 0.5% methyl cellulose and 0.025% Tween 20 to TRAMP mice beginning at 8 weeks of age for 20 weeks till experiment was terminated. (A) IHC of Ki-67 in the dorsolateral prostate from control and apigenin-treated TRAMP mice. A marked decrease in the expression of Ki-67 is observed after apigenin treatment. (B) Protein expression of Ki-67, cyclin D1, p27/Kip1 and BIM as determined by western blot analysis. A marked decrease in Ki-67 and cyclin D1 was noted after apigenin treatment along with increase in the protein expression of BIM and p27/Kip1. Numeric values represent the protein level normalized to the loading control (actin). Details are described in Materials and methods.