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. 2013 Sep 25;35(2):452–460. doi: 10.1093/carcin/bgt316

Fig. 5.

Fig. 5.

Effect of apigenin treatment on the protein expression of FoxO3a, p-FoxO3a (Ser253), Akt, p-Akt (Ser473) and 14-3-3 in human prostate cancer LNCaP and PC-3 cells. The cells were treated with 10 or 20 μM apigenin and 10 μM LY294002 for 16h and subjected to preparation of cytosolic and nuclear lysates. (A) A significant decrease in Akt and FoxO3a phosphorylation is observed along with increase in nuclear retention of FoxO3a after apigenin and LY294002 treatment. (B) FoxO3A DNA binding in the nuclear fractions. A significant increase in the nuclear FoxO3a binding was observed after apigenin treatment. **P < 0.001 control versus apigenin treatment. Mean ± SD of four samples. Details are described in Materials and methods.