Fig. 6.
Effect of apigenin on the binding of FoxO3a to p27/Kip1 and reduced proliferation of human prostate cancer LNCaP and PC-3 cells. The cells were treated with 20 μM apigenin and 10 μM LY294002 for 16h and subjected to preparation of nuclear lysate. (A) A marked increase in the binding of FoxO3a with p27/Kip1 was observed after apigenin treatment. Similar results were observed with LY294002. (B) Cell cycle analysis. The cells were serum starved for 36h followed by treatment with 10 and 20 μM apigenin and 10 μM LY294002 in RPMI-1640 complete media for 16h and subjected for cell cycle analysis. A significant increase in G0/G1 phase of the cell cycle was observed after apigenin and LY294002 treatment. Details are described in Materials and methods.