Figure 1. Loss of Trp53 in mMECs cultured in adhesive conditions delays their transition from a luminal to basal-like phenotype.

Freshly isolated Trp53^+/+ (a–h) and Trp53^−/− (i–p) primary mMECs were cultured in plastic dishes and sampled every three days. (a–d, i–l) Luminal differentiation was monitored by immunofluorescence staining for cytokeratin 18 (Krt18, green). (e–h, m–p) Basal differentiation was monitored by staining for ΔNp63 (red). Insets in (g, h, o, and p) demonstrate selected nuclei at a higher magnification. Arrowheads indicate ΔNp63 excluded from nucleoli. Arrows show ΔNp63 located in nucleoli. Scale bars correspond to 20 μm.