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. 2014 Apr 11;4:4663. doi: 10.1038/srep04663

Figure 3. Nucleolar localization of ΔNp63 correlates with EMT and functional inactivation.

Figure 3

(a) Phase contrast images (upper row) demonstrate an epithelial cobblestone-like (MCF10A and HCC1937) and mesenchymal (MDA-MB-231) morphology of human basal mammary cell lines. In cobblestone-like cells, ΔNp63 is excluded from nucleoli (arrowheads, second row). In mesenchymal-like cells, ΔNp63 is found in nucleoli (arrow). Functional inactivation of ΔNp63 using HDAC1 inhibitor trichostatin A (+TSA, third row) leads to a relocation of ΔNp63 into nucleoli in all cell lines. (b) MCF10A cells plated at a low density spontaneously undergo EMT and demonstrate a mesenchymal morphology. In these cells ΔNp63 is found predominantly in nucleoli (arrows). Insets demonstrate higher power views of selected cells in corresponding samples. (c) Co-staining of ΔNp63 with a nucleolar marker nucleophosmin (NPM) showing a nucleolar localization of ΔNp63 protein in MDA-MB-231, MCF7 and TP53−/− MCF10A cells, but not the parental MCF10A cells. Red, ΔNp63; blue, DAPI. Scale bars correspond to 20 μm in (a–b), and 10 μm in (c).