Figure 5.

Chromosomal recruitment of condensin I^DC and condensin II involves both shared and distinct regulators. (A) Motif logos of 10 bp DNA sequence motifs enriched at the top condensin sites are shown. (B) Overlap between binding sites of condensin I-I^DC, condensin II and SCC-2 is shown. Numbers under each factor indicate the total number of binding sites. Overlapping numbers are based on the number of SCC-2 peaks. (C) University of California Santa Cruz (UCSC) browser view exemplifying SCC-2 ChIP-seq signal, which is mostly restricted to promoters. (D) SCC-2 and condensin II binding at a well-defined condensin I^DC recruitment site on the X (rex-1). (E) In sdc-2 null mutant (TY1072), condensin I-I^DC (DPY-26), condensin II (HCP-6, KLE-2) and SCC-2 binding is diminished at rex-2 (left panel), but remains largely similar on autosomes (right panel). (F) Box plot of the ratio of ChIP enrichment in sdc-2 mutant versus wild type within the binding peaks of SCC-2. Binding sites are classified as being on autosomes, on the X with low SDC-2 binding and high SDC-2 binding. (G) qPCR analysis of DPY-27 ChIP enrichment in embryos isolated from adults that were fed vector (control) and PQN-85 RNAi. ChIP enrichment is expressed as relative to the negative control locus. Error bars are the standard deviations from three to five biological replicates. ChIP, chromatin immunoprecipitation; DCC, dosage compensation complex.