Figure 6.

Microinjection set up. The injection dish is clamped to the microscope stage with a minimum of water. The coarse adjustment is all that is needed to inject sea star eggs, while the fine adjustment is optional. The Femtojet is controlled by the mouse or by the foot pedal (not shown). To rapidly inject many eggs at a time, it is most efficient to align the needle in the field of view first. Once the pitch and position of the needle is setup, simply change the target by moving the stage control and the actual injection with the coarse z-axis control.