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. 2013 Dec 22;15(1):70–76. doi: 10.1002/embr.201337642

Figure 4. HIF predominantly regulates transcription through release of promoter-paused RNA-pol2.

Figure 4

  • A–C Mean distribution of normoxic (red) and hypoxic (blue) RNApol2 binding at the 100 transcripts most upregulated by hypoxia. Inset shows expanded view at the TSS (FPKM = fragments per kilobase per million reads). The same plots for (B) H3K4me3 and (C) DNAse1 hypersensitivity are shown.
  • D, E ChIP-qPCR analysis of RNApol2 within the body of (D) ALDOA and (E) NDRG1 gene shows suppression of the hypoxic induction by HIF-1α & HIF-2α siRNA (biological duplicates).
  • F Immunoblot analysis of HIF-α levels.
  • G,H RNA-seq and ChIP-seq tracks illustrating (G) release of promoter-paused RNApol2 and (H) de novo recruitment of RNApol2, despite constitutive DNAse1 hypersensitivity.