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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: J Am Chem Soc. 2013 Dec 16;136(1):188–194. doi: 10.1021/ja407858c

Fig. 2.

Fig. 2

Interfacial water and protein dynamics of HEWL-RC in D2O/glycerol mixtures. a, FFCFs for HEWL-RC in D2O/PEG mixtures, ranging from pure D2O to 80% PEG by volume. b, Hydration timescale, obtained by the initial decay of the correlation function, and the protein dynamics, estimated by the static offset of the correlation function, plotted as a function of solvent composition. A strong coupling is clear from the data, with both the hydration and protein dynamics slowing down as glycerol is added to the system. There is also a sharp dynamic transition occurring at roughly 60% PEG. We suggest this transition results from the extended protein hydration environment overlapping with the PEG hydration environment. c, The vibrational relaxation—estimated from the rephasing signal amplitude—lacks any PEG400 dependence suggesting that the protein remains fully hydrated in the region around the probe.