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Western blot detection of protein levels of SMADs and pSMADs expressed at the end of Stg2 or Stg3 in cells treated with DM, SB or DM/SB (A, B). All cell cultures exhibit similar levels of SMADs 1, 5, 8 and SMADs 2, 3. DM and DM/SB dramatically down-regulated pSMADs 1, 5, 8 while SB only partially lowered the levels of pSMADs 2, 3. (C, D) Quantification of western blot results shown in panel A, B.
