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. Author manuscript; available in PMC: 2014 Apr 11.
Published in final edited form as: Dev Biol. 2013 Jan 23;376(1):62–73. doi: 10.1016/j.ydbio.2013.01.012

Fig. 4.

Fig. 4

mRNA levels (A) and protein levels (B) of mDA markers examined at different stages after treatment of hES (H9 line) cells with DM, SB or DM/SB. At the end of Stg2, both SIP1 and Sfrp1 expression levels were increased after DM and DM/SB treatment. By mid-Stg3, Sfrp1 expression levels fell dramatically with DM and DM/SB treatment. At the end of Stg3, DM and DM/SB treatment greatly increased the expression of Wnt1 and Lmx1a (and somewhat increased Wnt3a and Wnt5a) while SHH expression decreased. At the end of Stg5, TH expression levels were increased with DM, SB and DM/SB treatment. (C) Quantification of Western blot results shown in panel B.