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Image of polyacrylamide gel for MPG mRNA expression in the 3D EpiDerm™ models, following two topical applications of MMS (n = 2). Lane 1: DNA ladder. Lane 2: empty. Lanes 3 and 4: 10 µl acetone. Lanes 5 and 6: 1000 µg/ml (31.25 µg/cm2). Lanes 7 and 8: 1900 µg/ml (59.375 µg/cm2). Lane 9: PCR positive control (β-actin). Lane 10: positive control, using RNA from TK6 cells. Lane 11: PCR negative control.
