Table I.
Standardisation of doses of MMC and MMS between 2D and 3D cell culture systems to correct for differences in dose regimes.
| MMC | MMS | ||||
|---|---|---|---|---|---|
| 2D | 3D (topical) | 2D | 3D (topical) | 3D (medium) | |
| LOGEL (µg/ml) | 0.01 | 4.8 | 0.5 | 1750 | 20 |
| Final concentration (µg/ml) | 0.01 | 9.6 | 0.5 | 3500 | 40 |
| Total µg applied to system | 0.1 | 0.096 | 5 | 35 | 36 |
LOGEL for MMC in 2D represents that of L5178Y tk+/− cells (25). LOGEL for 2D was multiplied by 10 to account for 10ml culture, whereas 3D doubled to account for the two doses administered during the RSMN assay. Adjustments for total mass (µg) applied to system due to two 10 µl applications on the topical surface and 900 µl volume of medium.