Fig. 3.

Viral antigen expression in tissues from CA16CC024-infected neonatal mice. The tissues were obtained on day 5 after intracerebral inoculation with medium or CA16CC024 at 10^3.5 CCID₅₀ ml⁻¹. Samples were embedded and frozen in liquid nitrogen, then fixed in 3.7 % paraformaldehyde and treated with hydrogen peroxide (2.5 %). Rabbit anti-CA16 antibody and HRP-conjugated anti-rabbit IgG antibody were used for immunohistochemical staining. Magnification:×400 (scale bars, 20 μm). The viral antigen was exhibited in the heart (b), intestine (d), liver (f), spinal skeletal muscle (h), lung (j), hind-limb muscle (l) and cortex of the brain (p) of infected mice, but not in the spleen (m) or brain medulla (o). In contrast, no viral antigen was detected in the heart (a), intestine (c), liver (e), spine skeletal muscle (g), lung (i), hind-limb muscle (k) or brain (n) of non-infected mice. The results are representative of three independent experiments.