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. 2014 May;95(Pt 5):1104–1116. doi: 10.1099/vir.0.060939-0

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — (a) Reactivity profile (ELISA) of rO1K-wt virus and its derivatives with neutralizing antigenic site-specific O Lausanne murine mAbs. Sucrose gradient-purified 146S antigens were used to ensure all the mutant viruses had an equivalent amount of antigen in the test. (b) ELISA results of individual and pooled guinea pig hyperimmune sera against rO1K-wt virus and its derivatives. Sucrose gradient-purified 146S antigens were used to ensure all the mutant viruses had an equivalent amount of antigen in the test. (c) Reactivity profile (ELISA) of the 5M virus when compared to the parent rO1K-wt virus using neutralizing antigenic site-specific O Manisa murine mAbs (SA85, SA107, SA113 and SA127) and O Lausanne bovine mAbs (C2, C96 and MH5). Sucrose gradient-purified antigens were used to ensure all the mutant viruses had an equivalent amount of antigen in the test.