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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Comp Neurol. 2013 Aug 1;521(11):2502–2522. doi: 10.1002/cne.23295

Figure 6.

Figure 6

Synaptic relationships between terminals immunostained for SP or Met-/Leu-Enk (immunogold) and ChAT-immunoreactive dendrites (immunoperoxidase) in the monkey putamen. A,B: Photomicrographs showing SP-ir boutons (+Ter) forming symmetric synapses (black arrows) with a small-sized and a medium-sized ChAT-labeled dendrite (A and B, respectively). C,D: Examples of Enk-ir terminals (+Ter) forming symmetric synapses (arrows) with a large-sized (C) and a medium-sized (D) ChAT-labeled dendrite. Note examples of unlabeled boutons (U Ter) forming asymmetric synapses (arrowheads) with spines (Sp) in the same fields of view. E: Mean percentages (± SEM) of ChAT-ir dendrites of different sizes in the double-immunostained tissue for SP/ChAT (n = 5 animals) or Enk/ChAT (n = 4 animals). F: Mean percentages (± SEM) of SP-positive and Enk-positive terminals in contact with ChAT-ir elements in the monkey putamen. *, P < 0.005. G: Mean percentages (± SEM) of SP- and Enk-ir terminals in contact with ChAT-immunoreactive soma and dendrites of various sizes. *, P = 0.012 Enk-labeled versus SP-labeled on large dendrites. Abbreviations: Den, dendrite. Scale bar = 0.5 μm in A,C; 0.2 μm in B,D.