. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: J Comp Neurol. 2013 Aug 1;521(11):2502–2522. doi: 10.1002/cne.23295

Table 1. Primary Antibodies Used in This Study.

Antibody	Immunogen	Manufacturer data	Dilution
Choline acetyltransferase	Purified human placental enzyme	Chemicon (AB143), rabbit polyclonal	1:2,000
Choline acetyltransferase	Purified human placental enzyme	Pro Sci (50–265), goat polyclonal	1:100
Substance P	Synthetic peptide corresponding to the full substance P protein conjugated to BSA	Millipore (MAB356), rat monoclonal	1:200
Methionine-enkephalin	Synthetic Met⁵-ENK peptide (H-TGGPM-OH) conjugated through the N-terminal tyrosine to BSA	Millipore (AB5026), rabbit polyclonal	1:1,000
Leucine-enkephalin	Synthetic Leu⁵-ENK peptide (H-TGGPL-OH) conjugated through the N-terminal tyrosine to BSA	Millipore (AB5024), rabbit polyclonal	1:1,000
γ-Aminobutyric acid	GABA-BSA	Sigma (A2052), rabbit polyclonal	1:1,000