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. Author manuscript; available in PMC: 2014 Apr 11.
Published in final edited form as: Innate Immun. 2013 Jun 28;20(3):269–282. doi: 10.1177/1753425913490534

Figure 7.

Figure 7

Negative-ion MALDI–MSn spectrum of hexaacyl lipid A at m/z 1772.2 from E. coli mutant strain MLK217 (lpxL) grown at 42°C. MSn spectra of hexaacyl lipid A species at m/z 1772.2 [4×14:0(3-OH), 14:0, 16:0] with the palmitic acid [16:0] in 2-O-position (on the proximal glucosamine). (A) MS2 of m/z 1772.2; (B) MS3 of m/z 1543.9 (selected from MS2 1772.2); (C) MS4 of m/z 1299.8 (selected from MS3 1543.9, selected from MS2 1772.2); (D) MS5 of m/z 1073.8 (selected from MS4 of 1299.8, selected from MS3 1543.9, selected from MS2 1772.2). Additional MSn spectra of this lipid A are provided in Supplementary Figure S7.