Fig. 4.

Inhibition of PQ²⁺-induced mitochondrial H₂O₂ by a representative glyoxylate metalloporphyrin. After the addition of PQ²⁺, H₂O₂ net production in mitochondria was monitored with addition of AEOL11207 (100 and 300 nM). AEOL11207 demonstrates a dose-dependent inhibition of mitochondrial net H₂O₂ production. Although exogenously added SOD at a saturating concentration (500 U/ml) had no effect on H₂O₂ net production, catalase is capable of scavenging H₂O₂, thus serving as a positive control. Similar results were obtained with other glyoxylate metalloporphyrins.