Biomechanical analysis indicates day 3 toroids can withstand greater deformation before failure and exhibit greater ultimate tensile strength than day 6 toroids. Treatment with Y-27632 significantly increased the displacement at failure of day 3 toroids as compared to day-matched control. Error bars indicate standard deviation. * indicates day 3 control ultimate tensile strength with respect to day 6 control and day 6 inhibitor-treated ultimate tensile strengths (two-way ANOVA, Bonferroni post-test, p<0.05); # indicates day 3 inhibitor-treated displacement at failure with respect to day 3 control and day 6 control displacements at failure, and day 3 inhibitor-treated ultimate tensile strength with respect to day 6 control and day 6 inhibitor-treated ultimate tensile strengths (two-way ANOVA, Bonferroni post-test, p<0.05).