Table 1. Primers used for quantitative real time polymerase chain reaction.
Primer sequences and concentrations used for quantitative real time polymerase chain reaction experiments (Exp. 2A and Exp. 3A).
| Primer | Sequence | Concentration |
|---|---|---|
| Cyclophilin | 5′-GTGTTCTTCGACATCACGGCT-3′ (forward) 5′-CTGTCTTTGGAACTTTGTCTGCA-3′ (reverse) |
200 nM |
| Enkephalin | 5′-GGACTGCGCTAAATGCAGCTA-3′(forward) 5′-GTGTGCATGCCAGGAAGTTG-3′ (reverse) |
100 nM |
| Galanin | 5'-TTCCCACCACTGCTCAAGATG-3' (forward) 5'-TGGCTGACAGGGTTGCAA-3' (reverse) |
100 nM |
| Melanin concentrating hormone | 5′-ATCGGTTGTTGCTCCTTCTCTG-3′ (forward) 5′-TCT GCT TGG AGC CTG TGT TCT T-3′ (reverse) |
100 nM |
| Neuropeptide Y | 5′-CACAGAAAATGCCCCCAGAA-3′ (forward) 5′-GTCAGGAGAGCAAGTTTCATTTCC-3′ (reverse) |
100 nM |
| Orexin | 5′-AGATACCATCTCTCCGGATTGC-3′ (forward) 5′-CCAGGGAACCTTTGTAGAAGGA-3′ (reverse) |
100 nM |