Table 6. Digoxigenin-labeled in situ hybridization in triglyceride subgroups.

High triglyceride rats show increased hypothalamic galanin but decreased orexin mRNA cell density, as assessed by digoxigenin-labeled in situ hybridization (Experiment 3B).

Subgroup	PVN GAL (cells/µm × 10−5)	PVN ENK (cells/µm × 10−5)	PFLH OX (cells/µm × 10−5)	PFLH MCH (cells/µm × 10−5)
Low	2.09 ± 0.03	2.74 ± 0.14	7.42 ± 0.22	8.44 ± 0.22
Middle	2.72 ± 0.07**	2.79 ± 0.05	6.31 ± 0.20**	8.25 ± 0.25
High	3.02 ± 0.16**	2.99 ± 0.13	6.54 ± 0.30**	8.29 ± 0.19

Values are mean ± S.E.M.

^**p < 0.01 vs. low subgroup.

See text or legend to Figure 3 for abbreviations.