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. 2014 Apr 11;9(4):e89502. doi: 10.1371/journal.pone.0089502

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© 2014 Malki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunodetection with anti-HasR (left panel) and anti-HasA (right panel) antibodies of whole cell extracts of S.marcescens SM365 either transformed with pBAD24 (control) or pBADHas_CTD for periplasmic expression of HasS_CTD. Lane 1, SM365pBAD24 grown in presence of glucose and iron; lane 2, SM365pBAD24 grown in presence of glycerol, dipyridyl and heme; lane 3, SM365pBAD24 grown in presence of arabinose, dipyridyl and heme; lane 4, SM365pBADHasS_CTD grown in presence of glucose and iron; lane 5, SM365pBADHasS_CTD grown in presence of glycerol, dipyridyl and heme; lane 6, SM365pBADHasS_CTD grown in presence of arabinose, dipyridyl and heme.