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. 2014 Apr 11;9(4):e93590. doi: 10.1371/journal.pone.0093590

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© 2014 Xue et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Assembly of endothelial “cell strings” over a large area of gelatin fibril pattern. (b) An image slice of the reconstructed 3-D conformation of a selected cell string. (c) to (f) are immunostaining images of, untreated ECs on a gelatin array (c), ROCK inhibited ECs with I(5 M) on a gelatin array (d), untreated ECs on bare PDMS (e), and ROCK inhibited ECs with I(5 M) on bare PDMS (f); (g) and (h) are the false color segmentation images for (e) and (f) respectively. Color code for immunostaining: blue = Hoechst stain, green = Phalloidin stain, and red = Vinculin anti-body stain.

Inline graphic — (a) Assembly of endothelial “cell strings” over a large area of gelatin fibril pattern. (b) An image slice of the reconstructed 3-D conformation of a selected cell string. (c) to (f) are immunostaining images of, untreated ECs on a gelatin array (c), ROCK inhibited ECs with I(5 M) on a gelatin array (d), untreated ECs on bare PDMS (e), and ROCK inhibited ECs with I(5 M) on bare PDMS (f); (g) and (h) are the false color segmentation images for (e) and (f) respectively. Color code for immunostaining: blue = Hoechst stain, green = Phalloidin stain, and red = Vinculin anti-body stain.