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. 2014 Apr 11;9(4):e94142. doi: 10.1371/journal.pone.0094142

Table 1. PCR primers.

Target Primer Sequence Size Temp. References
α2 5′-GTGGAGGAGGAGGACAGA-3′ (s) 155 bp 58 °C [31]
5′-CTTCTGCATGTGGGGTGATA-3′ (as)
α3 5′-CAAGCAACGAGGGCAACG-3′ (s) 121 bp 60 °C [32]
5′-CCGTCCTGGCAGGGGTAG-3′ (as)
α4 5′-GTTCCATGACGGGCGGGTGCAGTGGACT-3′ (s) 482 bp 60 °C [32]
5′-GGGATGACCAGTGAGGTGGACGGGATGAT-3′ (as)
α5 5′-TTTCTTCACACGCTTCCCAAA-3′ (s) 179 bp 60 °C [32]
5′-TCACGGACATCATTTTCCTTCA-3′ (as)
α6 5′-TCCATCGTGGTGACTGTGT-3′ (s) 125 bp 58 °C [31]
5′-AGGCCACCTCATCAGCAG-3′ (as)
α7 5′-GGGAACCTGCTGTACATCGGC-3′ (s) 115 bp 60 °C [32]
5′-GGTGCTCATCGTGCGTGGG-3′ (as)
α9 5′-GTCCAGGGTCTTGTTTGT-3′ (s) 403 bp 55 °C [33]
5′-ATCCGCTCTTGCTATGAT-3′ (as)
α10 5′-CTGTTCCGTGACCTCTTCG-3′ (s) 388 bp 60 °C Present Work
5′-GAAGGCCGCCACGTCCA-3′ (as)
β2 5′-CAGCTCATCAGTGTGCA-3′ (s) 347 bp 55 °C [33]
5′-GTGCGGTCGTAGGTCCA-3′ (as)
β3 5′-TGGGGAGTACCTGCTGTTCA-3′ (s) 385 bp 58 °C [30] Present Work
5′-CGGCATAATTGGGAATACCA-3′ (as)
β4 5′-AGCAAGTCATGCGTGACCAAG-3′ (s) 210 bp 60 °C [32]
5′-GCTGACACCTTCTAATGCCTCC-3′ (as)