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. 2014 Apr 11;9(4):e94551. doi: 10.1371/journal.pone.0094551

Figure 2. NPG-12 peptide selection and generation of anti-NPG-12 antibodies.

Figure 2

(A) hfgl2 antigenic index (Jameson-Wolf) and hydrophilicity plot (Hopp-Woods) analysis. Four serine peptidase Clan E catalyzing sites (SXXK motifs) were identified. The target peptide NPG-12 mapped to positions 76 to 87 near Ser91XXK is indicated by a red bar. (B) The titer of the anti-NPG-12 antibodies were increased to its peak in the NPG-12-KLH group after the third immunization, whereas no detectable titer was found in the normal and the PBS-KLH and peptide-pre-incubated groups, as determined by ELISA. Peptide incubated: peptide NPG-12 pre-incubated with anti-NPG-12 antisera group.