Table 1. Thermodynamic parameters of two Ca2+ binding sites in M-crystallin monomer and octamer.
Parameter | Monomer | Octamer |
Kd1 (µM) | 31±7 | 31±6 |
ΔH1 (kcal/mol) | −7.7±0.1 | −8.5±0.1 |
ΔS1 (cal/mol/K) | −5.2±0.8 | −7.9±0.8 |
ΔG1 (kcal/mol) | −6.2±0.2 | −6.2±0.2 |
Kd2 (µM) | 200±60 | 166±60 |
ΔH1 (kcal/mol) | −3.2±0.1 | −3.2±0.1 |
ΔS1 (cal/mol/K) | 6.2±1.0 | 6.6±1.1 |
ΔG2 (kcal/mol) | −5.0±0.2 | −5.2±0.2 |