Table 2. Mechanical properties of M-crystallin.

Sample	Contour length change (nm)†	Unfolding force (pN)*	Δxu (nm)	ku 0 (1/s)	ΔG‡ (kcal/mol)	ks (N/m)
Apo	29.1±0.7	91±21	0.55±0.02‡	1.1×10−2–1.0×10−4 ‡ a	16.4±1.4‡ b	0.75¶
	(n = 254)		0.49±0.02§	0.07±0.03§
Holo	28.9±0.6	125±20	0.38±0.02‡	1.1×10−2–5.0×10−4 ‡ a	15.9±0.9‡ b	1.52¶
	(n = 194)		0.34±0.02§	0.11±0.05§

^†The ‘n’ in the parentheses is the number of events used in the analysis.

*mean ± SD.

^‡Monte Carlo simulation.

^§Bell-Evans-Ritchie approximation.

^athe range is obtained by using the average unfolding force ± SD in Monte Carlo simulations (see Supporting Material in File S1).

^bthe errors obtained from the range of k_u ^o.

^¶k_s calculated from Monte Carlo values of k_u ⁰ and Δx_u.