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. 2014 Apr 11;9(4):e94487. doi: 10.1371/journal.pone.0094487

Figure 4. ALDH1 enzymatic activity and mRNA expression in thyroid cancer cell lines.

Figure 4

(A) ALDH1 enzymatic activities in four thyroid cancer cell lines were detected using the ALDEFLUOR assay. DEAB was used to inhibit the ALDEFLUOR reagent, providing a negative control. Filled histograms represent the distribution of ALDH1 enzymatic activities in each cell line in the absence of DEAB, open histograms show those in the presence of DEAB. (B) Relative mRNA expressions of ALDH1 in four thyroid cancer cell lines were quantitated by RT-PCR. Expression levels were normalized by β-actin.