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. 2014 Apr 11;9(4):e94382. doi: 10.1371/journal.pone.0094382

Figure 6. Effects of ATG9A and miR-34a expression on Ang II-induced autophagic activity in cardiomyocytes.

Figure 6

(A) Flow cytometry. The autophagic vacuoles were detected by using flow cytometry with an anti-autophagy marker protein (LC3) in the cardiomyocytes. The graph summarizes the data of flow cytometry. *P<0.05 compared with Control group. (B) Flow cytometry. The graph summarizes data from the flow cytometry. *P<0.05 compared with NC group, #P<0.05 compared to Ang II + NC. (C) Flow cytometry. The graph summarizes data from the flow cytometry. *P<0.05 compared with NC group, #P<0.05 compared to Ang II + NC. (D) TEM. The autophagic vacuoles were detected and calculated by transmission electron microscopy (as indicated by the arrows). Magnification of images: ×13500. The graph is the quantitative data from the transmission electron microscopy. Data are presented as means ± SEM *P<0.05 compared with Control group; #P<0.05 compared to Ang II + NC. (E) TEM. Magnification of images: ×13500 and ×46000, respectively. The graph is the quantitative data from the transmission electron microscopy. Data are presented as means ± SEM *P<0.05 compared with NC group; #P<0.05 compared to Ang II + NC.