Figure 5. MAP colony forming units derived from liver tissue of Rag2^−/− mice 8 weeks post infection.

Three different T cell subsets were used for adoptive transfer 4 weeks post infection: CD4⁺CD45RB^hi, CD4⁺CD45RB^lo/int and CD8⁺. Only infected as well as infected and reconstituted animals contained colony forming units i.e. MAP, as expected. Although all mice of a group were injected i.p. with 10⁸ MAP, plating 8 weeks later did not indicate a homogeneous infection. From some infected mice viable MAP could not be revealed from liver. Similar data were obtained using PCR. Each group n = 4 mice. The experiments were carried out twice with similar results.