Figure 2. Native field grown EV and irAOC leaf and root-associated bacterial communities (OTUs) are not influenced by developmental stages and jasmonic acid.

In the culture-independent approach, at the class level relative abundance of operational taxonomic units (OTUs) of field-grown N. attenuata plants is independent of the developmental stages and the ability of the plant to produce jasmonates (JA). JA-producing empty vector (EV) plants and plants impaired in JA biosynthesis by silencing allene oxide cyclase (irAOC) were grown in pairs at a field site in the plant's native habitat. Schematic representation of the plants' developmental stages at harvest (A). Abundance of bacterial composition at the class level in EV and irAOC leaves (B) and roots (C). For abbreviations see Figure 1. All samples were rarefied to 6374 sequences.