Figure 2.

Contralateral kidney of db RAS but not WT RAS or db sham mice developed chronic renal injury. A. Representative histological images of contralateral kidney at 4 weeks post-surgery as stained with periodic acid schiff (PAS), fibronectin (FN), and Masson’s Trichrome. PAS images are taken at 400× magnification. Fibronectin and trichrome images are taken at 200× magnification. B. Glomeruli of db RAS develop greater mesangial matrix expansion compared to both WT RAS and db sham mice at all time-points. Mesangial matrix expansion was assessed using the PAS sections with a 0 to 4 scale (see Methods). C. db RAS but not WT RAS mice develop de-novo fibronectin deposition within the glomeruli. Graph showed % fibronectin positive glomeruli to total number of glomeruli per histological sections in the db RAS mice. No fibronectin positive glomeruli were observed in the WT sham, WT RAS, or db sham. D. Interstitial fibronectin deposition is increased in db RAS compared to WT RAS or db sham mice at all time-points. Interstitial fibronectin deposition was assessed as percent area positive for fibronectin in each 200× field over the whole cortical areas. E. Interstitial fibrosis is increased to a greater extent in db RAS mice compared to WT RAS mice. Interstitial fibrosis was assessed using trichrome stained sections at 200× magnification. Data are presented as means ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. WT sham. § p < 0.05, §§ p < 0.01, §§§ p < 0.001 vs. WT RAS. ††† p < 0.001 vs. db sham. Statistical significance for B, D, and E was determined by 2-way ANOVA followed by Tukey adjusted post-hoc comparison. Statistical significance for C was determined by one way ANOVA followed by Tukey adjusted post-hoc comparison.