Figure 1.

Flagellin clone identity and similarity to known flagellin sequences. (A) Schematic of CBir flagellin clones from serological expression screening. The predicted amino acid sequences from the flagellin expression clones (CBir1_CBir15) are mapped in relation to the representation of the B. fibrisolvens sequence at the top. Ruler length equals 500 amino acids. Similarity in the NH₂ conserved sequence between these flagellin clones and B. fibrisolvens sequences ranged from 45 to 84% (mean, 60.3%). Breaks in the lines representing clones CBir1 and CBir2 indicate differences in sequence length in the hypervariable region. NH₂-conserved, conserved NH₂ sequence; CO₂H-conserved, conserved carboxy sequence. (B) Phylogenetic tree showing relatedness at the conserved NH₂ termini of CBir1_CBir15 clones to flagellin sequences in the GenBank database. The dendrogram was constructed using the Clustal program in DNAStar and reflects similarity at the amino acid level. The approximate location of the Clostridium subphylum cluster XIVa is indicated with a bracket.