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Iron ingestion induces urinary hepcidin in humans. Five subjects collected their first morning urine for 9 days and took 65 mg of iron (as ferrous sulfate) in the morning of days 3, 4, and 5. Thick line represents the arithmetic mean. There is a significant increase in hepcidin excretion on day 4 (24 hours after the first dose of iron) compared with day 3 (P = 0.017 by paired Student's t test).
