Figure 1. IgG and IgG-RNase constructs. (A) Schematic illustrations of IgG and IgG-RNase, as well as (B) the corresponding gene expression cassettes. In addition to IgG-RNase containing wild type human pancreatic RNase (RNase), a control construct is shown containing enzymatically inactive RNase variant RNase(H12A, H119A). The illustrations are not drawn to scale. attB1–2, BP recombination cloning sites; BGH Poly A, bovine growth hormone poly-adenylation signal; CMV, cytomegalovirus; RNase, human pancreatic ribonuclease; V, C, variable and constant regions of light (L) and heavy (H) IgG chain)