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Figure 2. SDS-PAGE of purified MN-IgGs and IgG-RNase constructs. A total of 1 µg purified protein per lane was tested by SDS-PAGE under non-reducing (lane 1–4) and reducing (lane 5–8) conditions followed by Coomassie staining. Lane 1 and 5: MN-IgG; lane 2 and 6: MN-IgG-RNase; lane 3 and 7: MN-IgG-RNase(H12A, H119A); lane 4 and 8: CTX-IgG-RNase; M: protein standard All Blue (BioRad)
