Figure 6. Internalization of fluorescently labeled MN-specific IgG-RNases. IgGs, IgG-RNases and control constructs were chemically conjugated with CypHer 5E and incubated for up to 24 h on MIAPaCa-MN⁺ cell overexpressing MN antigen. CTX-IgG-RNase was used as control. (A) Fluorescence microscopy was performed after different time points, images after 3 and 24 h are shown as examples. Hoechst 33342 was used to counter stain for nuclei. (B) Internalization was quantified by counting of red fluorescent granules per cell for up to 24 h.