Figure 7. Growth inhibition of MN⁺ overexpressing tumor cell lines. MN⁺ overexpressing MIAPaCa 2 cells were incubated (A) with MN-IgG-RNase containing catalytic active human pancreatic RNase. MN-IgG-RNase(H12A, H119A) with a catalytic inactive RNase, and CTX-IgG-RNase, were used as negative controls, whereas Onconase was used as non-targeted positive control. B) Additionally, MN⁺ overexpressing MIAPaCa 2 were also incubated with (B) MN-IgG based immunoRNase constructs fused with RI evasive human pancreatic RNase variants (Jo2007, QBI-119) as well as MN-IgG-RNase and MN-IgG-QBI119 constructs containing other linker sequences (GGFKGG, GGGGGG, GGFLGG, GGAANG, and GALALAG), which are putatively cleavable in endosomes. Free QBI119 and Onconase were also tested.