Table 1. Overview of the human IgGs and RNases used in this study.
IgG | Antigen specificity | Remarks |
---|---|---|
MN | MN-antigen (tumor associated antigen carbonic anhydrase IX, CA9) | Integral plasma membrane glycoprotein |
CTX | Cholera toxin (control antigen) | Soluble protein |
Mesothelin | Mesothelin-antigen (tumor associated antigen; MSLN) | GPI-anchored plasma membrane glycoprotein |
X | Undisclosed antigen X | GPI-anchored plasma membrane glycoprotein |
RNase | ||
RNase | Wild type human pancreatic RNase with truncated carboxy terminus (ΔSVEDST) for increased stability26 according to reference 23 | |
QBI-119 | Wild type human pancreatic RNase with truncated carboxy terminus (ΔSVEDST) and with mutations providing RI evasion (R4C, G38R, R39D, L86E, N88R, G89D, R91D, V118C) according to patent WO2005115477 | |
Jo2007 | Wild type human pancreatic RNase with truncated carboxy terminus (ΔSVEDST) and with mutations providing RI evasion (R39D, N67D, N88A, G89D, R91D)27 |
Abbreviations: GPI, glycophosphatidylinositol