Table 2. Binding kinetic parameters from SPR calculated by 1:1 Langmuir binding model.

Construct	ka (M−1 s−1)	kd (s−1)	KD (M)
MN-IgG1	2.6 × 105	7.1 × 10−4	2.7 × 10−9
MN-IgG1-RNase	2.7 × 105	6.5 × 10−4	2.4 × 10−9
MN-IgG1-RNase(H12A, H119A)	2.7 × 105	6.8 × 10−4	2.5 × 10−9