Table 3. Catalytic efficiency of purified immunoRNases and control constructs.
| Construct | Production system | Catalytic efficiency kcat/Km per RNase moiety [M−1 s−1] |
|---|---|---|
| MN-IgG-RNase | HEK293 | 1.4 × 108 |
| MN-IgG-RNase(H12A, H119A) | HEK293 | < 104 * |
| CTX-IgG-RNase | HEK293 | 7.0 × 107 |
| Mesothelin-IgG-RNase | HEK293 | 1.1 × 108 |
| X-IgG-RNase | HEK293 | 1.9 × 108 |
| MN-IgG1-QBI119 | HEK293 | 5.2 × 107 |
| MN-IgG1-Jo2007 | HEK293 | 9.1 × 107 |
| QBI119 | E. coli | 4.1 × 106 |
| Onconase | E. coli | 4.6 × 103 |
*Catalytic efficiency below detection limit at the maximal tested concentration of 1010 M.