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. 2004 May 1;113(9):1334–1343. doi: 10.1172/JCI21252

Figure 4.

Figure 4

p57 expression is upregulated in the absence of PTHrP in vivo. (A and B) Sections from control and PTHrP_/_ littermates. (A) In situ hybridization showing p57 mRNA expression in E15.5 tibia. The PTHrP-null tibia shows upregulation of p57 in the columnar proliferative chondrocytes (c), which are dramatically reduced in the PTHrP-null tibia. (B) BrdU/p57 double-immunofluorescent detection in E14.5 tibia. In controls, proliferating chondrocytes are labeled with BrdU (green), and p57 protein (red) is upregulated in prehypertrophic and hypertrophic chondrocytes. In the PTHrP-null tibia, p57 is upregulated in the columnar proliferative chondrocytes, interspersed among BrdU-positive cells. (C_F) Sections of E18.5 ulna from control, p107_/_; p130_/_, and PTHrP_/_; p107_/_; p130_/_ littermates. (C) In situ hybridization showing p57 mRNA expression. The PTHrP/p107/p130-null ulna shows upregulation of p57 in the proliferative chondrocytes (p). (D) In situ hybridization showing Ihh mRNA expression. (E) In situ hybridization showing PTH/PTHrP receptor expression. The PTHrP/p107/p130-null tissue does not show upregulation of Ihh or PTH/PTHrP receptor in the proliferative chondrocytes, indicating there is a specific increase in p57 expression. (F) BrdU/p57 double-immunofluorescent detection. In the PTHrP/p107/p130-null tissue, p57 protein (red) is upregulated in the proliferative chondrocytes. Original magnification, ∞10 in all panels.