Figure 4. Characterization of the clones selected during MS2. (A) Ratio obtained by phage-ELISA for the best clones of MS1 and MS2 compared with the positive control Fc-QL. (B) Sequence analysis of the 219 positive clones (ratio/Fc-WT > 2), distribution of the mutations along the Fc region revealing 9 key positions, which are mutated in more than 10% of the positive clones. (C) Sequences of the 10 best variants isolated during MS2. Key positions as identified in Figure 4B are in bold.