Table 2. Comparison of binding of IgG variants to FcRn using 3 different techniques: ELISA on coated FcRn, FcRn binding on cells and SPR on immobilised FcRn.

EC₅₀: Concentration at 50% saturation obtained from the ELISA binding curve of each IgG variant. The ratio is the EC₅₀ of the IgG-WT divided by the EC₅₀ of the variant. IC5₀: Concentration at 50% inhibition of the MFI signal obtained with Alexa-conjugated Rituximab alone (100%). The ratio is the IC₅₀ of the IgG-WT divided by the IC₅₀ of the variant.