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Figure 2. Apparent affinity (A) and neutralization potency (B) of Fab fragments expressed in E. coli. CMV strain VR1814 used as source of antigen and virus for binding and neutralization assays. The single mutants T55R, D53M, D53Q, D53T and D53S were not tested in neutralization assays. Only the neutralization data with Fab at concentration of 0.64 nM of the 5-fold dilution series between 0.128 and 400 nM is shown. Average and range of duplicate neutralization assays are shown.
