Skip to main content
. 2014 Jan 16;6(2):437–445. doi: 10.4161/mabs.27875

Table 1. Binding kinetics of Fab fragments with different residues in heavy chain positions 52, 53 and 55 to CMV strain VR1814 gH/gL.

Variant Sequencea kon (M−1s−1)b koff (s−1)b KD (pM)
MSL-109     NSDST 5.2 × 106 5.2 × 10−4 100
D53N/T55R     NSNSR 1.0 × 107 6.0 × 10−5 < 10c
Germline (IGHV3–21)     SSSSS 4.6 × 106 2.6 × 10−4 57
Asp-53 only     SSDSS 2.9 × 106 4.6 × 10−4 160
Thr-55 only     SSSST 2.3 × 106 7.7 × 10−4 330

aSequence between heavy chain Kabat positions 52 and 55 shown. The second residue is position 52a. b Binding kinetics measured at 37 °C. c Upper-limit of estimated KD shown due to kon and koff values close to or above the detection limit.