Figure 3. Kinetic binding and limited neutralizing activity of group 1 and group 2 HA cross-reactive mAb 486. (A) Comparison of the binding kinetics of mAb 486 after capture on an anti-human Fc sensors, to 200 nM of HA trimers representative of group 1 (H1 California 07/09 and H5 Vietnam/1203/2004) and group 2 (H3 Hong Kong/8/68 and H7 Netherlands/219/03). (B) Plaque formation neutralization activity of mAb 486, rabbit pAb directed to the fusion peptide, or mAb 579 of H1 A/CA/04/09 or H3 A/Perth/16/09 infected MDCK cells, with (black) or without (blue) prior trypsin activation of viruses.