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. 2004 May 1;113(9):1266–1270. doi: 10.1172/JCI21301

Figure 1.

Figure 1

Hepatic differentiation after transplantation of a single HSC. Fourteen months after transplantation of a single β-gal+ HSC, random liver sections were analyzed by X-gal staining. (A) Cells with hepatocyte morphology (large cells with abundant cytoplasm) that clearly expressed β-gal and were integrated into the liver parenchyma were detected in primary single-cell recipients. The micrographs were taken at ∞20 magnification, with the inset at ∞100. Clonally derived hematopoietic BM cells were harvested from primary recipients and transplanted into Fah_/_ mice. (B) Five months after transplantation, whole-mount X-gal staining on liver lobes revealed extensive blue nodular repopulation (arrowheads). (C) FAH immunohistochemistry (dark brown staining) shown on a liver section confirms widespread oligoclonal repopulation throughout the host parenchyma (arrowheads). (D) Increased magnification of a repopulated nodule reveals typical hepatocyte morphology of the brown FAH+ cells. (E) Frozen serial sections stained with X-gal and anti-FAH show colocalization of staining, confirming that the FAH expression is derived from the original Rosa26 (lacZ+) HSC.