Table 3. Kinetics parameters of the interactions of chimeric constructs with mAb measured by QCM.

Constructs	H27K15a
	ka (105.M−1.s−1)	kd* (10−2.s−1)	KD (nM)
mCD115 (WT)	NB	NB	NB
2/3D1 mutD2	6.2 ± 0.03	1.0 ± 0.01	16 ± 0.1
hCD115 (WT)	5.8 ± 0.05	0.9 ± 0.01	16 ± 0.1
1/3D1	3.2 ± 0.30	5.4 ± 0.01	168 ± 20.0
2/3D1	2.8 ± 0.90	4.5 ± 0.01	158 ± 50.0
hD1	3.4 ± 1.00	3.8 ± 0.01	109 ± 0.9

^a Binding of H27K15 to CD115 constructs. Deduced from curves presented in Figure 7B. *Error considered as 0.01 when error from the fitting model was found below 0.01. NB: No detectable binding